Comments on: Interrogation of a conserved overlap between cofactor and substrate binding domains to facilitate crosstalk-induced conformational changes in Class A Flavin Monooxygenases: A case study of 6-Hydroxynicotinate-3-Monooxygenase

By: Sam Belsky

Sam Belsky — Fri, 21 Apr 2023 21:42:23 +0000

Successfully performing the regiospecific hydroxylation of an aromatic ring is very difficult primarily because the use of any aqueous solvent and the addition of a catalyst can lead to multiple undesired hydroxylations. Of course the addition of certain functional groups to the aromatic ring may help direct a hydroxylation, but since a catalyst is still required for this type of reaction, additional hydroxylations are likely to occur. Stereospecific hydroxylations of aromatic rings require the dearomitization of the aromatic ring, to generate a chiral carbon bound to 4 distinct atoms. This would mean that the aromatic reactant has a functional group present at the carbon in question prior to reaction, making the addition of another functional group unfavorable due to steric restraints. On top of this, aromatic rings tend to be very stable, making it more likely that if an aromatic compound is hydroxylated, it’s much more likely to occur at any carbon with no functional group.

By: Khallyani Minich

Khallyani Minich — Fri, 21 Apr 2023 21:00:29 +0000

Can you comment on why it is to hard to sterically/Regio-selectively hydroxylate an aromatic ring with traditional organic chemistry methods?

By: Sam Belsky

Sam Belsky — Fri, 21 Apr 2023 02:34:17 +0000

I think at least with regard to my IS – and this was true with both the introduction and the discussion especially, the most difficult aspect of composing my thesis was finding the most effective, succinct, and straight forward way in which to communicate my ideas. The topic of my thesis was extremely technical, so finding a way to logically lay out both the rationale for my research and what I ultimately found took a while.

By: Charitha Patlolla

Charitha Patlolla — Fri, 21 Apr 2023 00:35:18 +0000

What was the most difficult part of writing an IS?