Comments on: An Examination of Antibiotic Resistance Genes in Pseudomonas chlororaphis in Response to Bacteriophage Therapy /2021/04/03/eric-kraus-21/ Thu, 04 May 2023 18:10:58 +0000 hourly 1 https://wordpress.org/?v=6.9.4 By: Eric Kraus /2021/04/03/eric-kraus-21/#comment-10860 Sat, 17 Apr 2021 04:08:59 +0000 https://news.wooster.edu/?p=7238#comment-10860 In reply to Thinh Tan Huynh.

Thinh, thank you for the questions. The antibiotic susceptibility testing of the parent strain occurred by taking 3 control trials (bacteria without antibiotic) and 3 experimental trials (bacteria + one specific antibiotic) and then measuring the growth afterwards. Due to the time it took to allow for the bacteria to grow and the number of antibiotics used, testing had to occur over weeks, therefore leading to multiple control trials being grown on different days.
As for the phase II experiment, the difference between the red and the purple originates from differences in mutant growth in that antibiotic. Red indicates that the mutant simply grew worse than the parent strain in the antibiotic (OD units/mL difference for the mutant > OD units/mL difference for the parent strain). The purple also indicates that the mutant grew less in the antibiotic compared to the parent strain, but by a greater difference. If the mutant grew twice as less in antibiotic compared to the control, then it was labelled purple instead of red. I hope this answers your questions!

]]> By: Eric Kraus /2021/04/03/eric-kraus-21/#comment-22926 Sat, 17 Apr 2021 04:08:59 +0000 https://news.wooster.edu/?p=7238#comment-22926 In reply to Thinh Tan Huynh.

Thinh, thank you for the questions. The antibiotic susceptibility testing of the parent strain occurred by taking 3 control trials (bacteria without antibiotic) and 3 experimental trials (bacteria + one specific antibiotic) and then measuring the growth afterwards. Due to the time it took to allow for the bacteria to grow and the number of antibiotics used, testing had to occur over weeks, therefore leading to multiple control trials being grown on different days.
As for the phase II experiment, the difference between the red and the purple originates from differences in mutant growth in that antibiotic. Red indicates that the mutant simply grew worse than the parent strain in the antibiotic (OD units/mL difference for the mutant > OD units/mL difference for the parent strain). The purple also indicates that the mutant grew less in the antibiotic compared to the parent strain, but by a greater difference. If the mutant grew twice as less in antibiotic compared to the control, then it was labelled purple instead of red. I hope this answers your questions!

]]> By: Eric Kraus /2021/04/03/eric-kraus-21/#comment-22925 Sat, 17 Apr 2021 03:58:45 +0000 https://news.wooster.edu/?p=7238#comment-22925 In reply to Stephanie Strand.

Thank you Dr. Strand! I would not have been able to do it without all of your help along the way. I’m excited to see what the new students find!

]]> By: Eric Kraus /2021/04/03/eric-kraus-21/#comment-10859 Sat, 17 Apr 2021 03:58:45 +0000 https://news.wooster.edu/?p=7238#comment-10859 In reply to Stephanie Strand.

Thank you Dr. Strand! I would not have been able to do it without all of your help along the way. I’m excited to see what the new students find!

]]> By: Stephanie Strand /2021/04/03/eric-kraus-21/#comment-22924 Fri, 16 Apr 2021 20:59:48 +0000 https://news.wooster.edu/?p=7238#comment-22924 Congratulations Eric! You have done great work this semester, and there are a couple of students who are excited to continue the investigations!

]]> By: Stephanie Strand /2021/04/03/eric-kraus-21/#comment-10858 Fri, 16 Apr 2021 20:59:48 +0000 https://news.wooster.edu/?p=7238#comment-10858 Congratulations Eric! You have done great work this semester, and there are a couple of students who are excited to continue the investigations!

]]> By: Thinh Tan Huynh /2021/04/03/eric-kraus-21/#comment-22923 Fri, 16 Apr 2021 18:38:20 +0000 https://news.wooster.edu/?p=7238#comment-22923 In reply to Thinh Huynh.

Also, best luck on your journey after 糖心Vlog传媒.

]]> By: Thinh Tan Huynh /2021/04/03/eric-kraus-21/#comment-10857 Fri, 16 Apr 2021 18:38:20 +0000 https://news.wooster.edu/?p=7238#comment-10857 In reply to Thinh Huynh.

Also, best luck on your journey after 糖心Vlog传媒.

]]> By: Thinh Huynh /2021/04/03/eric-kraus-21/#comment-22922 Fri, 16 Apr 2021 18:35:12 +0000 https://news.wooster.edu/?p=7238#comment-22922 Congrats on your study Eric. This is an amazing work. I have a few questions. What accounted for the different growths in all the controls in phase I? Since the controls were grown without any anti-biotic, should all of them be the same? In phase II, what is the difference between the color red and purple given that these strains all grew less than the parent strain?

]]> By: Thinh Huynh /2021/04/03/eric-kraus-21/#comment-10856 Fri, 16 Apr 2021 18:35:12 +0000 https://news.wooster.edu/?p=7238#comment-10856 Congrats on your study Eric. This is an amazing work. I have a few questions. What accounted for the different growths in all the controls in phase I? Since the controls were grown without any anti-biotic, should all of them be the same? In phase II, what is the difference between the color red and purple given that these strains all grew less than the parent strain?

]]>